Excellent System Expandability
Solvent Delivery Unit Accommodates a Wide Range of Recovery Volumes
Guidelines for Preparative Scale and Maximum Load
The figure at right shows the guidelines for total component capacity with a 250 mm long column when the target component is highly soluble in the mobile phase, separates from impurities, and ions are suppressed. For isocratic elution, in principle, the total component capacity is proportional to the column volume.
Guidelines for Preparative Scale and Maximum Load
- This solvent delivery unit can handle flow rates ranging from those used in analytical scale to those used in semi-preparative (up to 10 mL/min).
- High-precision analysis is possible even in the semi-micro flow-rate range.
Supports Semi-Preparative and Recycle Preparative
- This solvent delivery unit can handle flow rates used in semi-preparative scale (up to 20 mL/min).
- Using a recycle kit enables semi-preparative recycling.
Supports Large-Scale Preparative Fractionation
- High flow rates (up to 150 mL/min) enable highly efficient, large-scale preparative fractionation.
- Large-scale prep solvent delivery fully supports the preparative fractionation workflow including reinjection to assess purity.
- Combine with an FCV-200AL low-pressure gradient unit to perform gradient analysis using up to four mobile phases.
|Solvent delivery method||Series-type double plunger||Parallel-type double plunger|
|Plunger capacity||Primary side: 47 μL, Secondary side: 23 μL||47 μL||250 μL|
|Maximum discharge pressure||40 MPa||49 MPa||42 MPa|
|Flow rate setting range||0.001 to 10.000 mL/min||0.001 to 20.000 mL/min||0.01 to 150.00 mL/min|
|Flow rate accuracy||
No more than ±2% or ±2 μL/min,
whichever is greater (0.01 to 5 mL/min)
No more than ±1% or ±10 μL/min,
whichever is greater (0.1 to 5.0 mL/min)
No more than ±1% (1 mL/min, 10 MPa)
|Flow rate precision||No more than 0.06% RSD or 0.02 min SD,
whichever is greate
|No more than 0.08% RSD or 0.02 min SD, whichever is greater||No more than 0.1% RSD or 0.02 min SD,
whichever is greater
|Constant pressure solvent delivery||Supported|
|Plunger rinsing mechanism||Syringe or rinsing pump||Syringe or rinsing pump|
|Operating temperature range||4 to 35 °C||4 to 35 °C||4 to 35 °C|
|Size and weight||W 260 x H140 x D 420 mm，11 kg||W 260 x H140 x D 500 mm，16 kg||W 260 x H210 x D 500 mm，19 kg|
Shim-pack Scepter Columns
- Excellent stability & performance using a wide range of LC conditions -
Shim-pack Scepter LC columns, which are the next generation organic silica hybrid based columns, are designed for stability and performance in a wide range of mobile phase conditions. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting under conditions that may compromise traditional silica based columns.
With different particle sizes (1.9 μm, 3 μm, 5 μm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC, making method transfer seamless between different laboratory instrumentation.
|Shim-pack Scepter||Reversed Phase|
|Functional Group||Trifunctional C18||Trifunctional C18||Trifunctional C8||Trifunctional
|Generic Purpose Type||High Density Type|
|Particle||Organic Silica Hybrid|
|Particle Size||1.9 μm，3 μm，5 μm|
|Pore Size||12 nm||8 nm||12 nm|
|pH Range||1 - 12||1 - 10||1 - 8|
|100% Aqueous Condition||Yes||No||No||Yes||Yes|
The LH-40 Liquid Handler, Combination of Autosampler and Fraction Collector
|Maximum Number of Containers||Up to 540 pcs* (10 mm I.D. test tubes)||Up to 100 pcs* (1.5 mL vials)|
|Maximum Injection Volume||Up to 20 mL (with the Large Capacity Injection Kit)||Up to 5 mL|
|Cooling Function||None||Yes (Optionally available)|
|Compatible Containers and Quantities||
10 mmO.D. test tube 540 pcs
12 mmO.D. test tube 486 pcs
16 mmO.D. test tube 216 pcs
18 mmO.D. test tube 216 pcs
25 mmO.D. test tube 108 pcs
35 mmO.D. test tube 54 pcs
MTP/DWP 9 pcs
1.5 mL vials 100 pcs
4.0 mL vials 80 pcs
13 mL vials 25 pcs
|Compatible Containers and Quantities||W 390 x H 560 x D 730 mm, 40 kg||W 260 x H 280 x D 320 mm, 18.5 kg|
* Depends on the type of racks used
FRC-40, Highly Flexible Fraction Collector
Compact Design for Small-Volume Samples
For smaller scale collection, or specialized applications that require enclosure and cooling, the FRC-10A is a compact fraction collector that provides time and signal based triggering. A variety of programmable fractionation functions enable target components to be collected with high purity and high recovery.。
|Maximum Number of Fractions||Up to 540 pcs*||Up to 144 pcs*|
|Maximum Flow Rate||150 mL/min|
|Fractionation Mode||Configured through a combination of basic mode (initial parameter mode) and time program mode (14 parameters)|
|Cooling Function||None||Optionally available|
|Compatible Containers and
10 mmO.D. test tube 540 pcs 4 mL vial 252 pcs
12 mmO.D. test tube 486 pcs 15 mL vial 108 pcs
16 mmO.D. test tube 216 pcs 50 mL vial 54 pcs
18 mmO.D. test tube 216 pcs 250 mL vial 20 pcs
25 mmO.D. test tube 108 pcs 500/1000 mL bottle 12 pcs
35 mmO.D. test tube 54 pcs MTP/DWP 9 pcs
10 mmO.D. test tubes 144 pcs
18 mmO.D. test tubes 164 pcs
35 mmO.D. test tubes 16 pcs
|Size and weight||W390 × H560 × D730 mm, 30 kg||W 260 x H 280 x D 420 mm，15 kg|
Example of Scaling Up from Analytical to Semi Preparative Work
This is an example of scaling up in which seven types of parabens are targeted using a 150 mm long column with a particle size of 5 μm.
The gradient elution conditions investigated at the analytical scale are transitioned to the semi-preparative scale. A comparable chromatogram is obtained at both scales.
Column: Shim-pack Scepter C18-120 (4.6 mm x 150 mm，5 μm）
Column: Shim-pack Scepter C18-120 (20 mm x 150 mm，5 μm）